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Lipopolysaccharide (LPS) is a major virulence factor of Gram-negative bacteria that triggers activation of numerous signaling pathways and is known to modulate expression of many genes in various cell types. Human beta-defensins are a family of antimicrobial peptides highly produced in human epithelial cells and skin. In order to analyze their regulation, we studied the effects of LPS stimulation on expression of HBD-1, -2, and -3 mRNAs in several human peripheral blood cell types and skin microvascular endothelial cells. Flow cytometric and PCR analysis showed that HBD-1 and -2 are induced in monocytes, granulocytes, T-cells and keratinocytes, whereas HBD-3 is expressed only